Product Name

Peroxidase from horseradish

General deion

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca₂₊(~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Synonym

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

Assay

200-300 units/mg solid (using pyrogallol) Highly stabilized, essentially salt-free

CAS Number

9003-99-0

MDL number

MFCD00071339

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Brown to Very Dark Brown and Red to Very Dark Red and Red-Brown lyophilized powder

l Biological Information

Biochem/Physiol Actions

Horseradish peroxidase has been shown to catalyze the polymerization of various substances including acetaminophen and cardanol.

Application

Peroxidase from horseradish has been used in a study with dehydrogenative polymers (DHP) to investigate the monolignol coupling mechanism.It has also been used to help develop a colorimetric method for microRNA (miRNA) detection by creating a horseradish peroxidase-mimicking DNAzyme.

Preparation Note

Stabilized by chemical protection of the primary amines.

Caution

Maintains activity at low pH and higher temperature.

Unit Definition

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov