Product Name

Myeloperoxidase from human leukocytes

General deion

Myeloperoxidase (MPO) is encoded by the gene mapped to human chromosome 17q22-24.The encoded protein is a leukocyte-derived enzyme and is an integral constituent of the innate immune response. MPO belongs to the heme peroxidase superfamily. MPO is a heme protein containing iron and is highly expressed in leukocytes, neutrophils, monocytes and some subtypes of tissue macrophages.

Synonym

MPO, Peroxidase, myelo

Assay

≥50 units/mg protein

CAS Number

9003-99-0

MDL number

MFCD00131641

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Light Green lyophilized powder

Solubility(25°C)

H²O: soluble aqueous buffers such as 50 mM sodium acetate: soluble (Freezing of the enzyme in solution may result in a substantial loss of activity.)

Physical form

Lyophilized from 50 mM sodium acetate buffer, pH 6.0, 0.1 M sodium chloride

l Biological Information

Biochem/Physiol Actions

Myeloperoxidase (MPO) has a crucial role to play in destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells and malignant and nonmalignant nucleated cells.MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease.MPO catalyze the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursor such as chlorophenols in presence of hydrogen peroxide (H²O²).

Application

Myeloperoxidase (MPO) from human leukocytes has been used in MPO assay.

Unit Definition

One unit of myeloperoxidase will catalyze the consumption of one micromole of hydrogen peroxide and the production of ¹/₄ micromole of tetraguaiacol per minute at pH 7.0 and 25 °C

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov