Product Name

Chloroperoxidase from Caldariomyces fumago

Synonym

Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase

Assay

1,000-2,000 units/mg protein (E_1%/₂₈₀)

CAS Number

9055-20-3

MDL number

MFCD00130774

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous suspension

Physical form

Purified suspension in 0.1 M sodium phosphate solution, pH approx. 4.5

l Biological Information

Biochem/Physiol Actions

Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme. It also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H²O² decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs).

Application

A useful alternative to lactoperoxidase for ₁₃₁I ion labeling studies, for bromination of proteins, and for ₃₆Cl labeling of macromolecules in long-term isolation procedures.

Unit Definition

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H²O².

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov