Product Name

Chloroperoxidase from Caldariomyces fumago

Synonym

Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase

Assay

≥3,000 units/mL

CAS Number

9055-20-3

MDL number

MFCD00130774

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous suspension

Physical form

Crude suspension in 0.1 M sodium phosphate, pH ~4.5

l Biological Information

Biochem/Physiol Actions

Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme and it also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H²O² decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs). PAHs are considered to be a potential health risk because of their possible carcinogenic and mutagenic activities and are widely dispersed in the environment.Chloroperoxidase is a heme containing glycoprotein that is secreted from fungus. It is similar magnetic and spectroscopic properties of cyctochrome P-450. Chloroperoxidase can catalyze the halogenations of organic substrates.

Application

A useful alternative to lactoperoxidase for ₁₃₁I ion labeling studies, for bromination of proteins, and for ₃₆Cl labeling of macromolecules in long-term isolation procedures.

Chloroperoxidase is a useful alternative to lactoperoxidase for ₁₃₁I ion labeling studies, for bromination of proteins, and for ₃₆Cl labeling of macromolecules in long-term isolation procedures. It has been used to study biocatalytic oxidation in polymersome nanoreactors.It has been used to study biocatalytic oxidation in polymersome nanoreactors.

Unit Definition

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H²O².

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov