Product Name

Catalase−Agarose enzyme from bovine liver

Synonym

H²O²:H²O² Oxidoreductase

Assay

1900-3800 units per ml of agarose

Molecular Weight

tetramer mol wt ~250 kDa

MDL number

MFCD00081483

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

Physical form

Suspension in 3.2 M (NH⁴)²SO⁴, pH7, containing 0.02% thimerosal

l Biological Information

Biochem/Physiol Actions

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu₂₊ results in degradation of the catalase molecule.

Application

Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria. Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H²O²) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing. Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H²O²) that can degrade the target product.

Caution

Freezing of solutions is not recommended.

Unit Definition

One unit will decompose 1.0 μmole of H²O²per min at pH 7.0 at 25 °C, while the H²O² concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov