Product Name

Catalase from human erythrocytes

Synonym

H²O²:H²O² oxidoreductase

Assay

≥90% (SDS-PAGE);  ≥30,000 units/mg protein

CAS Number

9001-05-2

Molecular Weight

tetramer mol wt ~250 kDa

MDL number

MFCD00081483

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

Physical form

solution in 50 mM Tris, pH 8.0

l Biological Information

Biochem/Physiol Actions

Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene  and apoptosis.

Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu₂₊ results in degradation of the catalase molecule.

Unit Definition

One unit will decompose 1.0 μmole of H²O²per min at pH 7.0 at 25 °C, while the H²O² conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A²⁴⁰.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov