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Catalase from bovine liver

Product #: S0083
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Details

l General Information

Product Name

Catalase from bovine liver

Synonym

H2O2:H2O2 oxidoreductase

Assay

40,000-60,000 units/mg protein (E1%/405)

CAS Number

9001-05-2

Molecular Weight

tetramer mol wt ~250 kDa

MDL number

MFCD00081483

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Brown to Dark Green aqueous suspension

l Biological Information

Biochem/Physiol Actions

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene  and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Preparation Note

This product is offered as a catalase suspension in water containing 0.1% (w/v) thymol with activity of 40,000-60,000 units/mg protein. To remove the thymol preservative, the catalase crystals may be pelleted by centrifugation, the supernatant, discarded, the pellet resuspended in water, and then pelleted again. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

Caution

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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