l General Information |
Product Name | Catalase from bovine liver |
Synonym | H2O2:H2O2 oxidoreductase |
Assay | ≥30,000 units/mg protein | CAS Number | 9001-05-2 |
Molecular Weight | tetramer mol wt ~250 kDa | MDL number | MFCD00081483 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | aqueous solution |
l Biological Information |
Biochem/Physiol Actions | Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate. |
Application | Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli. |
Preparation Note | This product is an aqueous solution containing >30,000 units/mg protein. To remove the thymol preservative, the catalase crystals may be pelleted by centrifugation, the supernatant, discarded, the pellet resuspended in water, and then pelleted again. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0. |
Caution | Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity. |
Unit Definition | One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240. |
l Storage |
Storage temp. | 2-8°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
