l General Information |
Product Name | Catalase from bovine liver |
General deion | CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons. |
Synonym | H2O2:H2O2 oxidoreductase |
Assay | 2,000-5,000 units/mg protein; suitable for cell culture | CAS Number | 9001-05-2 |
Molecular Weight | tetramer mol wt ~250 kDa | MDL number | MFCD00081483 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | Dark Green to Very Dark Green and Dark Beige to Very Dark Beige to Very Dark Brown Powder |
l Biological Information |
Biochem/Physiol Actions | Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate. |
Application | Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli. Catalase from bovine liver is used for the following applications: • Gel filtration • Analytical filtration on Ultrogel and glycerol gradient centrifugation • Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor • Enzyme assays • Alcohol oxidase activity |
Preparation Note | This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0. |
Caution | Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity. |
Unit Definition | One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240. |
l Packaging & Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
