Product Name

Catalase from Aspergillus niger

Synonym

H²O²:H²O² oxidoreductase

Assay

≥4,000 units/mg protein

CAS Number

9001-05-2

Molecular Weight

tetramer mol wt ~250 kDa

MDL number

MFCD00081483

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

Physical form

Suspension in 3.2 M (NH⁴)²SO⁴ solution, pH 6.0

l Biological Information

Biochem/Physiol Actions

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu₂₊ results in degradation of the catalase molecule.

Application

Catalase from Sigma has been used as a positive control during the functional characterization of Clostridium difficile spore coat proteins.

Caution

Freezing of solutions is not recommended.

Unit Definition

One unit will decompose 1.0 μmole of H²O² per min at pH 7.0 at 25 °C, while the H²O² concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A²⁴⁰.

l Packaging & Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov