Product Name

D-Fructose Dehydrogenase from Gluconobacter industrius

Synonym

D-Fructose:(acceptor) 5-oxidoreductase

Assay

400-1,200 units/mg protein

CAS Number

37250-85-4

MDL number

MFCD00130905

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Pink-Orange Lyophilized Powder

Physical form

Lyophilized powder containing citrate-phosphate buffer salts, TRITON® X-100, and stabilizer

l Biological Information

Biochem/Physiol Actions

D-fructose dehydrogenase catalyzes the oxidation of D-fructose to 5-keto-D-fructose.Fructose dehydrogenase (FDH) is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

Application

D-fructose dehydrogenase is used as a biosensor to detect the presence of D-fructose.Fructose dehydrogenase (FDH) is used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis.D-fructose dehydrogenase has been used in a study that contributed towards a convenient method for measuring rare sugars, monosaccharides, for applications in the bio-industry. A direct electron transfer reaction of d-fructose dehydrogenase adsorbed on a porous carbon electrode surface has been used to describe a batch-type coulometric d-fructose biosensor.

Unit Definition

One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37 °C.

l Packaging & Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov