Product Name

D-Fructose Dehydrogenase from Gluconobacter sp.

Assay

≥20 units/mg solid

CAS Number

37250-85-4

Molecular Weight

mol wt ~140 kDa

MDL number

MFCD00130905

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

supplied as a lyophilized powder containing approx 80% stabilizers, sugars, amino acids and BSA

l Biological Information

Biochem/Physiol Actions

D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

Application

This enzyme is also used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis.This enzyme is useful for enzymatic determination of D-fructose in clinical analysis.

Unit Definition

One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov