D-Fructose Dehydrogenase from Gluconobacter sp.
Product #:
S0073
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SKU-Pack Size
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Price($)
Quantity
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S0073-250UN
In-stock
1167.4
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Details
l General Information |
Product Name | D-Fructose Dehydrogenase from Gluconobacter sp. |
Assay | ≥20 units/mg solid | CAS Number | 37250-85-4 |
Molecular Weight | mol wt ~140 kDa | MDL number | MFCD00130905 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
Physical form | supplied as a lyophilized powder containing approx 80% stabilizers, sugars, amino acids and BSA |
l Biological Information |
Biochem/Physiol Actions | D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively. |
Application | This enzyme is also used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis.This enzyme is useful for enzymatic determination of D-fructose in clinical analysis. |
Unit Definition | One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37 °C. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |