Product Name

Cholesterol Oxidase microbial

Synonym

Cholesterol: oxygen oxidoreductase

Assay

≥10 units/mg protein recombinant

CAS Number

9028-76-6

Molecular Weight

mol wt 55 kDa

MDL number

MFCD00130783

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Solubility(25°C)

50 mM potassium phosphate buffer, pH 7.0: soluble

l Biological Information

Biochem/Physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H²O². Optimum pH of the enzyme is 7.0. Hg₂₊, Ag₊, ionic detergents inhibit the enzyme activity.

Application

Cholesterol oxidase is used to determine serum cholesterol.The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids.Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

Preparation Note

Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.

Unit Definition

One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase ed system.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov