Product Name

Glucose Oxidase from Aspergillus niger

Synonym

β-D-Glucose:oxygen 1-oxidoreductase, G.Od., Gox

Assay

100,000-250,000 units/g solid (without added oxygen)

CAS Number

9001-37-0

MDL number

MFCD00131182

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Faint Yellow to Dark Yellow lyophilized powder

Solubility(25℃)

1 mg/mL in 0.05 M Sodium Acetate;pH 5.1

l Biological Information

Biochem/Physiol Actions

Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.

Molecular Weight: 160 kDa (gel filtration) pI: 4.2 Extinction coefficient: E_1% = 16.7 (280 nm) Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-ed glycosylation.Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates. The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a K_M of 33-110 mM. Glucose oxidase does not require any activators, but it is inhibited by Ag₊, Hg₂₊, Cu₂₊, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.

Application

Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.

Preparation Note

This enzyme is soluble (1.0 mg/mL) in 50 mM sodium acetate buffer, pH 5.1, yielding a clear solution.

Unit Definition

One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H²O² per min at pH 5.1 at 35 °C, equivalent to an O² uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.

l Storage

Storage temp.

-20℃

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov