l General Information |
Product Name | Xanthine Oxidase (XOD) from cow milk |
General deion | Xanthine:oxygen oxidoreductase |
Assay | ~1 units/mg protein (At 25 °C with xanthine as the substrate.) |
Contaminants | :<0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate); Note: Chromatographically purified. |
Optimum pH | 8.5-9.0 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | ammonium sulfate suspension |
Physical form | Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8. |
l Biological Information |
Biochem/Physiol Actions | Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium. |
Application | Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR. Xanthine Oxidase has been used to study tyrosine nitration. |
Preparation Note | Activator: O2 Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added. |
Unit Definition | One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E293nm = 12.2 mmol -1 x L x cm-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5. |
l Packaging & Storage |
Storage temp. | -20℃ |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
