Product Name

Choline Oxidase from Alcaligenes sp.

Synonym

Choline: oxygen 1-oxidoreductase

Assay

≥10 units/mg solid

CAS Number

9028-67-5

Molecular Weight

mol wt ~95 kDa

MDL number

MFCD00130787

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as the intermediate and molecular oxygen as th eprimary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the study of the reaction mechanism for the conversion of choline to the aldehyde intermediate, & of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approx. 72,000 Da (gel filtration) or 66,000 Da (SDS gel electrophoresis).

Application

Choline oxidase from Alcaligenes sp. has been used in a study to investigate a choline biosensor constructed with chitinous membrane and its application in measuring cholinesterase inhibitory activities. Choline oxidase from Alcaligenes sp. has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.Choline oxidase from Alcaligenes sp. has been used to evaluate choline biosensors constructed with chitinous membranes. The enzyme has also been used to analyse the photoreaction of choline oxidase with covalently bound flavin, using spectroscopic techniques. Furthermore, the product can be used for cholinesterase activity and phospholipid determination assays.

Preparation Note

Solutions of choline oxidase may be prepared in 10 mM Trizma^@-HCl, pH 8.0, with 2.0mM EDTA and 134 mM KCl. One publication cites preparation of 2 mg/mL stock solutions of choline oxidase in carbonate buffer.

Unit Definition

One unit will form 1 μmole of H²O² with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by chr every μmole of choline.oline oxidase, 2 μmoles of H²O² are produced fo

l Storage

Storage temp.

-20℃

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov