Product Name

Alcohol Oxidase from Candida boidinii

Synonym

Alcohol:oxygen oxidoreductase

Assay

5-15 units/mg protein

CAS Number

9073-63-6

MDL number

MFCD00130452

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Yellow to Orange Lyophilized Powder

Solubility(25℃)

100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)

Physical form

Contains potassium phosphate buffer salts, DTE, and stabilizer

l Biological Information

Biochem/Physiol Actions

Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5.

Application

Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes. Product A6941 is from Candida boidinii and is provided as a lyophilized powder.

Unit Definition

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

l Packaging & Storage

Storage temp.

-20℃

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov