Product Name

Alcohol Oxidase solution from Pichia pastoris

Synonym

Alcohol:oxygen oxidoreductase

Assay

10-40 units/mg protein (biuret)

CAS Number

9073-63-6

MDL number

MFCD00130452

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

Physical form

Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0

l Biological Information

Biochem/Physiol Actions

Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.

Application

Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial  and immunological verification of Hv-p68 cDNA clones.

Preparation Instructions

Alcohol oxidase is soluble in cold 100 mM potassium phosphate, pH 7.5, at 25 °C. Solutions should be prepared just before use.

Unit Definition

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

l Packaging & Storage

Storage temp.

-20℃

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov