Product Name

Alcohol Oxidase from Hansenula sp.

Synonym

Alcohol:oxygen oxidoreductase

Assay

≥0.6 units/mg solid

CAS Number

9073-63-6

Molecular Weight

~600 kDa

MDL number

MFCD00130452

Suitability

BioReagent

l Physical and Chemical Information

Appearance

vacuum-dried powder

l Biological Information

Biochem/Physiol Actions

Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm.Catalyzes the oxidization of primary alcohols to aldehydes.

Molecular Weight: ~600 kDa

Optimum pH: 8.5

pH Range: 5.5-8.5

K_m values with the following substrates:

Allyl alcohol 33.3 mM

Benzyl alcohol 26.6 mM

Ethanol 16.2 mM

Formaldehyde 2.6 mM

Methanol 2.15 mM

N-Butanol 166 mM

n-Propanol 66 mM

Cofactor: FAD

Inhibitors: 1,4-butynediol (irreversible), propargyl alcohol (irreversible), cyclopropanol, cyclopropanone (suicide substrate), formaldehyde, H²O², hydroxylamine, KBr, KCN, methanol (substrate inhibitor), NaN³, PCMB, propynal, urea, 4-chloromercuribenzoic acid

Application

Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes.It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes.

Preparation Note

buffered aqueous glycerol solution Alcohol oxidase is soluble (0.1 unit/ml) in cold 100 mM potassium phosphate, pH 7.5 at 25 °C. Solutions should be prepared just before use.

Unit Definition

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

l Packaging & Storage

Storage temp.

-20℃

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov