Product Name

6-Phosphogluconic Dehydrogenase from yeast

Synonym

6-Phospho-D-gluconate:NADP₊ 2-oxidoreductase (decarboxylating), 6-Phosphogluconic Dehydrogenase from yeast

Assay

≥3.0 units/mg solid

CAS Number

9073-95-4

MDL number

MFCD00130317

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Enzyme activity is determined by measuring the reduction of NAD₊ or NADP₊. High levels of NADPH are believed to inhibit the enzyme, while 6-phosphogluconate acts to activate the enzyme.

Application

6-phosphoglyconic dehydrogenase (6PGD) is a key enzyme in the oxidative portion of the hexose monophosphate shunt. It is specific for oxidized nicotinamide adenine dinucleotide phosphate (NADP₊) . 6-phosphogluconate dehydrogenase is involved in the production of ribulose 5-phosphate, which is involved in nucleotide synthesis and the pentose phosphate pathway by generating NADPH. 6-phosphogluconate dehydrogenase is used to study nucleotide synthesis, glucose metabolism, and the protection of cells from oxidative damage

Unit Definition

One unit will oxidize 1.0 μmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate and CO² per min at pH 7.4 at 37 °C in the presence of NADP₊.

l Packaging & Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov