Product Name

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

General deion

D-lactic dehydrogenase catalyzes the conversion of D-lactate into D-pyruvate while reducing NAD₊ to NADH and H₊.

Synonym

(R)-3-Hydroxybutanoate:NAD₊ oxidoreductase, 3-HBDH

Assay

≥200 units/mg protein

CAS Number

9028-38-0

MDL number

MFCD00130633

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Unit Definition

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

l Packaging & Storage

Storage temp.

-20℃

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov