Ascorbate Oxidase from Cucurbita sp.
Product #：
S0006
Image
SKU-Pack Size
Stock
Price($)
Quantity
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S0006-100UN
In-stock
110.6
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S0006-250UN
In-stock
220.8
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S0006-1KU
In-stock
608.4
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Details
l General Information |
Product Name | Ascorbate Oxidase from Cucurbita sp. |
General deion | Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. |
Synonym | L-Ascorbate:oxygen oxidoreductase, Ascorbase |
Assay | 1,000-3,000 units/mg protein | CAS Number | 9029-44-1 |
MDL number | MFCD00130560 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa. |
Application | Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis . |
Preparation Note | The enzyme is reconstituted in 4 mM sodium phosphate buffer, pH 5.6, with 0.05% (w/v) bovine serum albumin for the Sigma enzymatic activity assay. |
Unit Definition | One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C. |
l Storage |
Storage temp. | -20℃ |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
