Product Name

Nitroreductase from Escherichia coli

General description

Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.

Assay

≥90%(SDS-PAGE), recombinant, expressed in E. coli

Molecular Weight

monomer mol wt 24000

MDL number

MFCD03457727

Suitability

BioReagent

l  Physical and Chemical   Information

Appearance

Faint Yellow to Yellow Powder

l  Biological Information

Biochem/Physiol   Actions

Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Application

Nitroreductase has been used in a study that used a set of PCR primers to clone a gene encoding a hypothetical nitroreductases (named as Ssap-NtrB) from uropathogenic staphylococcus. It has also been used to improve prodrug activation. Nitroreductase from Escherichia coli has been used in a study to assess anaerobic bacteria as a gene delivery system for cancer treatment. It has also been used in a study to investigate its applications in antibody-directed enzyme prodrug therapy.

Preparation   Note

Produced using animal component-free materials.

Unit   Definition

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

l  Storage

Storage   temp.

-20℃

l  Precautions and Disclaimer

l  References

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov